Coupling Methodology within the Software Platform Alliances

CEA, ANDRA and EDF are jointly developing the software platform ALLIANCES which aim is to produce a tool for the simulation of nuclear waste storage and disposal repository. This type of simulations deals with highly coupled thermo-hydro-mechanical and chemical (T-H-M-C) processes. A key objective of Alliances is to give the capability for coupling algorithms development between existing codes. The aim of this paper is to present coupling methodology use in the context of this software platform.Comment: 7 page

Effets de stimulations physiologiques et sécrétagogues sur le pH intracellulaire et les transports ioniques des cellules submandibulaires de souris SCA-9 (études cinétiques par vidéomicrofluorimétrie)

MONTPELLIER-BU Médecine (341722104) / SudocMONTPELLIER-BU Médecine UPM (341722108) / SudocREIMS-BU Santé (514542104) / SudocPARIS-BIUM (751062103) / SudocPARIS-BIUP (751062107) / SudocSudocFranceF

Coupled chemo-transport-mechanical modelling and numerical simulation of external sulfate attack in mortar

International audienceWe develop and apply in this study a chemo-transport-mechanical model for simulating the external sulfate attacks in Portland (CEM I) cement pastes and mortars. Basically, this degradation consists in the simultaneous decalcification of the hydrated phases resulting from leaching processes, and the migration of sulfate ions within the material and its subsequent interactions with these phases. The sulfate uptake leads generally to ettringite precipitation mainly from monosulfate, which in turn may produce intense macroscopic expansions and cracking. In our approach, crystallization pressures arising from the restrained growth of monosulfate crystals due to the confinement of the surrounding C–S–H matrix are assumed to initiate the observed macroscopic expansions. A macroscopic strain tensor evaluated from the volume fraction of supplementary precipitated ettringite is further introduced in the mechanical behavior law for explicitly reproducing the macroscopic expansions. Analytical homogenization schemes are applied to estimate both mechanical and diffusive properties from the local volume fraction of solid phases. The numerical platform Alliances is then used for solving both reactive transport and mechanical coupled problems, and is applied to the simulation of laboratory tests consisting in prismatic mortar specimens immersed in solutions containing sodium sulfate and subjected to free expansions. Comparison of the numerical results with experimental ones in terms of phase assemblage profiles, evolutions of mass changes and expansions shows a correct agreement. Finally, the extension of the model towards cases of restrained displacement conditions is discussed and some modifications regarding the kinetics of ettringite precipitation are proposed for such situations

Preproinsulin I and II mRNA expression in adult rat submandibular glands

Mammalian salivary glands are known to produce a number of biologically active peptides. The aim of this study was to extend our previous results showing the presence of a biologically active insulin‐like immunoreactive peptide in rat salivary glands. In rodents, where two nonallelic and functional insulin genes are expressed, the co‐expression of both genes seems to be limited to β‐cells of pancreatic islets or to embryologic developmental processes. We have investigated the expression of insulin genes in rat submandibular glands and in a murine immortalized submandibular cell line, SCA‐9. For this purpose, total RNAs were isolated and submitted to reverse transcription. The cDNAs obtained were amplified by a nested polymerase chain reaction using rat preproinsulin I and II primers. Our data show that both preproinsulin I and II mRNAs are expressed in adult rat submandibular glands as well as in the SCA‐9 cell line. The identification of salivary gland rat preproinsulin I and II was confirmed by direct sequencing. These results provide, for the first time, evidence for the expression of both preproinsulin I and II mRNA in an extra‐pancreatic tissue from adult rodents

Active Fault System across the Oceanic Lithosphere of the Mozambique Channel: Implications for the Southern Part of the East African Rift System

International audienceSeismic reflection and multibeam echosounder data acquired in the Mozambique Channel during the PAMELA project (PAssive Margin Exploration Laboratories, collaboration between IFREMER-TOTAL-IFPEN-CNRS-Univ. Brest, Rennes, Paris) revealed that an active fault system is deforming the oceanic lithosphere of the Mozambique Basin which has developed during Jurassic-Cretaceous times. The correlation between the fault system and the arrangement of earthquake epicenters suggests that this tectonically active zone directly connects northward with the southern part of the eastern branch of the East African Rift System which corresponds to the seismically active graben system bounding the northern part of the Davie ridge. The fault zone extends southwestward of the Mozambique Ridge along the same trend as the Agulhas-Falkland transform fault zone. The general organization of the fault zone shows the characteristics of an extensional system north of the Mozambique Channel (north of the Europa Island) and a right-lateral transtensional system with coeval normal faults and strike-slip faults south-west of Europa. This tectonic activity is associated with volcanic activity since at least Late Miocene times. Our findings emphasize that the eastern branch of East African Rift System is extending largely toward the south, not only in continental margin domains but also through the oceanic lithosphere of the Mozambique Basin. This fault zone is participating to the complex plate boundary between the main African continent (Nubia Plate) and the Somalia Plate

Active fault system across the oceanic lithosphere of the Mozambique Channel: Implications for the Nubia–Somalia southern plate boundary

International audienceSeismic reflection and multibeam echosounder data were acquired in the Mozambique Channel in 2014 and 2015 during the PTOLEMEE, PAMELA-MOZ02 and -MOZ04 marine surveys aboard the RV Atalante and Pourquoi Pas? These data revealed that an active fault system is deforming the oceanic lithosphere of the Mozambique Basin which has developed during Jurassic-Cretaceous times. The correlation between the fault system and the arrangement of earthquake epicenters suggests that this tectonically active zone directly connects northward with the southern part of the eastern branch of the East African Rift System which corresponds to the seismically active graben system bounding the northern part of the Davie ridge. The fault zone extends southwestward of the Mozambique Ridge along the same trend as the Agulhas-Falkland transform fault zone. The general organization of the fault zone shows the characteristics of an extensional system north of the Mozambique Channel (north of the Europa Island) and a right-lateral transtensional system with coeval normal faults and strike-slip faults south-west of Europa. This tectonic activity is associated with volcanic activity since at least Late Miocene times. Our findings emphasize that the eastern branch of East African Rift System is extending largely toward the south, not only in continental domains but also through the oceanic lithosphere of the Mozambique basin. This fault zone is participating to the complex plate boundary between the main African continent (Nubia Plate) and Madagascar (Somalia Plate)

Deciphering Black Extrinsic Tooth Stain Composition in Children Using Metaproteomics

The present study focuses on the use of a metaproteomic approach to analyze Black Extrinsic Tooth Stains, a specific type of pigmented extrinsic substance. Metaproteomics is a powerful emerging technology that successfully enabled human protein and bacterial identification of this specific dental biofilm using high-resolution tandem mass spectrometry. A total of 1600 bacterial proteins were identified in black stain (BS) samples and 2058 proteins in dental plaque (DP) samples, whereas 607 and 582 human proteins were identified in BS and DP samples, respectively. A large diversity of bacteria genera (142) in BS and DP was identified, showing a high prevalence of Rothia, Kingella, Neisseria, and Pseudopropionibacterium in black stain samples. In this work, the high diversity of the dental microbiota and its proteome is highlighted, including significant differences between black stain and dental plaque samples